The International Spy Museum is a privately owned museum dedicated to the field of espionage located in the Penn Quarter neighborhood of Washington, D.C., across the street from the Old Patent Office Building (which houses the Smithsonian American Art Museum and the National Portrait Gallery) and one block south of the Gallery Place-Chinatown Metro station.

The museum was built by The Malrite Company at a cost of US$40 million. Despite being one of the few museums in Washington that charges admission fees, it has been immensely popular since its opening in July 2002.

The over 600 artifacts in over 20,000 square feet (1900 m²) of exhibition space[3] relate the history of espionage and spies in real-world practice and in popular culture. The museum has extensive exhibits on espionage methods and materials dating from the Greek and Roman empires, the British empire, the American Revolutionary War, the American Civil War, the World Wars, and the Cold War years.

Most of the exhibit areas bring visitors into the era's espionage environment. Numerous visitor-interactive stations are located throughout the exhibit areas. At the terminus of the exhibit area an 8-minute feature film updates and describes how modern technology has changed the face of warfare and terrorism.